Har Gobind Khorana also known as
Hargobind Khorana was a biochemist who shared the 1968
Nobel Prize for Physiology or Medicine with
Marshall W. Nirenberg and
Robert W. Holley for research that helped to show how the
nucleotides in
nucleic acids, which carry the
genetic code of the cell, control the cell’s synthesis of proteins. Khorana and Nirenberg were also awarded the
Louisa Gross Horwitz Prize from
Columbia University in the same year natural causes he was 89..
[4]
He became a naturalized citizen of the United States in 1966,
[1] and subsequently received the
National Medal of Science. He served as
MIT's Alfred P. Sloan Professor of Biology and Chemistry, Emeritus
[5] and was a member of the Board of Scientific Governors at
The Scripps Research Institute.
(January 9, 1922 – November 9, 2011)
[2][3]
Early life and education
Khorana was born to
Hindu[6] parents in Raipur village in
West Punjab, British India, currently Pakistan.
[7][8]
His father was the village "patwari" (or taxation official). He was
home schooled by his father until high school. He earned his B.Sc from
Punjab University, Lahore, in 1943, and his M.Sc from
Punjab University,
Lahore, Pakistan in 1945. In 1945, he began studying at the
University of Liverpool. After earning a Ph.D in 1948, he continued his
postdoctoral studies in
Zürich (1948–1949). Subsequently, he spent two years at
Cambridge University. In 1952 he went to the
University of British Columbia, Vancouver and in 1960 moved to the
University of Wisconsin–Madison. In 1970 Khorana became the
Alfred Sloan Professor of Biology and Chemistry at the
Massachusetts Institute of Technology where he worked until retiring in 2007.
[9]
Khorana married Esther Elizabeth Sibler, of Swiss origin, in 1952.
[10]
They had three children: Julia Elizabeth (born May 4, 1953), Emily Anne
(born October 18, 1954; died 1979), and Dave Roy (born July 26, 1958).
[10]
Research work
Ribonucleic acid (RNA) with three repeating units (UCUCUCU → UCU CUC UCU) produced two alternating
amino acids. This, combined with the
Nirenberg and Leder experiment, showed that UCU codes for
Serine and CUC codes for
Leucine.
RNAs with three repeating units (UACUACUA → UAC UAC UAC, or ACU ACU
ACU, or CUA CUA CUA) produced three different strings of amino acids.
RNAs with four repeating units including UAG, UAA, or UGA, produced only
dipeptides and
tripeptides thus revealing that UAG, UAA and UGA are
stop codons.
[citation needed]
With this, Khorana and his team had established that the mother of all
codes, the biological language common to all living organisms, is spelled out in three-letter words: each set of three
nucleotides codes for a specific amino acid. Their
Nobel lecture was delivered on December 12, 1968.
[11] Khorana was the first scientist to synthesize
oligonucleotides.
[12]
Subsequent research
He extended the above to long DNA Polymers using non-aqueous chemistry and assembled these into the first synthetic gene, using
polymerase and
ligase enzymes that link pieces of DNA together.
[13] as well as methods that anticipated the invention of
PCR.
[14] These custom-designed pieces of artificial genes are widely used in biology labs for sequencing,
cloning
and engineering new plants and animals. This invention of Khorana has
become automated and commercialized so that anyone now can order a
synthetic gene from any of a number of companies. One merely needs to
send the
genetic sequence to one of the companies to receive an oligonucleotide with the desired sequence.
His lab has since mid 1970s
[15] studied the biochemistry of the membrane protein
bacteriorhodopsin
responsible for converting photon energy into proton gradient energy
and most recently studying the structural related visual pigment
rhodopsin.
[16]
Legacy
The
University of Wisconsin-Madison, the Government of India (DBT
Department of Biotechnology), and the Indo-US Science and Technology Forum jointly created the
Khorana Program
in 2007. The mission of the Khorana Program is to build a seamless
community of scientists, industrialists, and social entrepreneurs in the
United States and India.
The program is focused on three objectives: Providing graduate and
undergraduate students with a transformative research experience,
engaging partners in rural development and food security, and
facilitating public-private partnerships between the U.S. and India. In
2009, Khorana was hosted by the Khorana Program and honored at the 33rd
Steenbock Symposium in
Madison, Wisconsin.
[citation needed]
Death
Khorana died of natural causes on November 9, 2011 in
Concord, Massachusetts, aged 89.
[17] A widower, he was survived by his children Julia and Dave.
[18]
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